![]() The vertical scale of the chromatogram can be adjusted by clicking and dragging on the graph itself. Note that since the distance between bases as inferred from the trace varies the trace may be either contracted or expanded compared with the raw data. ![]() scf file.Ĭhromatogram peaks for individual bases can be turned off by checking the A/G/C/T boxes in the Graphs tab. Geneious does not perform base-calling itself: this information is already contained in the. Base calling may also assign a quality measure for each such call, typically in terms of the expected probability of making an erroneous call. ![]() The raw trace is processed by a “Base Calling” software which detects peaks in the four traces and assigns the most probable base at more or less even intervals. The raw output of a sequencing machines is known as a trace, a graph showing the concentration of each nucleotide against sequence positions. If the chromatograms are not visible, check Chromatograms under the Graphs tab (see Figure 5.6Ĭhromatogram files are produced from sequencing machines such as the Applied Biosystems 3730 DNA analyzer. ![]() Geneious can view chromatogram information from files imported in.
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